Use 10x Phosphate Buffered Saline (PBS) for washing western blots and as a wash buffer in other applications. When using alkaline phosphatase as a reporter in western blots, it is recommended that Phosphate Buffered Saline is replaced with Tris Buffered Saline (TBS). Evangeline Parish Library 916 W. Main St. Ville Platte, LA 70586 TEL: 337.363.1369 FAX:337.363.2353 Site Admin
The 10X NEBNext® Library Dilution Buffer is optimized for dilution of libraries to the appropriate concentration range for quantitation using the NEBNext Library Quant Kit for Illumina® (NEB #E7630). This buffer pack can be used to supplement the buffer volume supplied in the NEBNext® Library Quant Kit for Illumina®.
For information on importing the file into Local Run Manager, see the Add a Library Prep Kit section in the Local Run Manager Software Guide. View Options Local Run Manager Library Prep Kit Definition File for Nextera DNA Flex – Set A, 2 x 151, 96 Samples (iSeq and MiSeq)